2015年江伯倫老師實驗室論文成果發表II
 

2015年江伯倫老師實驗室論文發表--簡芊卉

Single allergen-induced oral tolerance inhibits airway inflammation in conjugated allergen immunized mice.

Chien CH1, Yu HH2, Chiang BL3.

Oral tolerance has been considered as the result of a number of protective mechanisms. However, the detailed mechanisms of oral tolerance are yet to be defined especially the non-antigen-specific regulation. We aimed to study whether a single allergen-induced oral tolerance would modulate the immune responses to other related allergens that were primed together with the orally treated allergen. We investigated whether oral administration of a single antigen would inhibit the other antigen induced airway inflammation in conjugated antigen-sensitized mice. We found that oral administration of ovalbumin (OVA) was able to prevent the immune responses to β-lactoglobulin (BLG) in BLG-conjugated OVA (B-O) sensitized mice and vice versa. Oral administration of antigen improved the suppressive potential of CD4+CD25+ T cells to inhibit non-antigen-specific T cell proliferation. We found that B cell-induced regulatory T (referred to as Treg-of-B) cells showed several similarities with orally treated CD4+CD25+ T cells, including the expression of inducible T-cell co-stimulator (ICOS) and programmed cell death 1 (PD1), the production of IL-10 and TGF-β, and the non-antigen-specific suppressive ability and might play a role in the sophisticated mechanism of oral tolerance. These results suggested that non-antigen-specific regulatory effect of oral tolerance could be detected in the condition that active Treg cells and effector T cells concurrent in the same position; thus, non-antigen-specific regulation of oral tolerance could more clearly detected in conjugated antigen-sensitized mice.

2015 May 26. pii: S0091-6749(15)00578-3. doi: 10.1016/j.jaci.2015.04.018. [Epub ahead of print]